Excited to say that we have draft genome assemblies for nine anthozoans! I used bbmerge and bbduk (https://sourceforge.net/projects/bbmap/) to clean and trim sequences. DiscovarDenovo (https://www.broadinstitute.org/software/discovar/blog/) was used for the draft assemblies. Computation time on nine species was ~1 week (on a 512 GB RAM 64 processor). That is all!
We are currently comparing the draft assemblies to other assemblers (SOAPdenovo, SPAdES) and I am also blasting the contigs (and also the trimmed reads) for environmental contaminants. This latter step is the tricky part. If you are reading this and have any advice, please send it my way.
Finally, we have sequenced Renilla on PacBio as well. I will be coupling these data with the Illumina data to generate a hyrbid assembly. Exciting! Stay tuned for more~
UCE Project Team
All things Anthozoa, Evolution and Ecology
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